Journal: Science Advances

Article Title: A multiomics atlas of the Arabidopsis nuclear envelope

doi: 10.1126/sciadv.aee7658

Figure Lengend Snippet: ( A ) Arabidopsis root schematic is colored according to annotated cell types derived from stage-resolved root scRNA-seq analyses ( GSE152766 ), with the corresponding UMAP shown on the left. The UMAP shown on the right is a projection of the same dataset ordered along a pseudotime trajectory (T0, youngest/least differentiated cells; T9, most mature/differentiated cells) illustrates developmental progression from the meristematic niche to differentiated tissues. ( B ) z score–normalized expression heatmap of NPC and PNET genes across pseudotime. ( C ) Coexpression modules identified by hdWGCNA are mapped to the pseudotime-ordered UMAP project in (A). Two major modules (M1 and M2) are shown, each enriched for distinct functional categories. Gene counts for NPC and PNET genes in each module are shown on the far right. ( D ) Representative confocal images showing spatial distribution of Nup promoter activity ( pNup::NLS-GFP ) and corresponding Nup proteins ( pNup::gNup-GFP ) in transgenic Arabidopsis root. The quiescent center is indicated by an arrowhead. Scale bars, 100 μm. ( E ) Cycloheximide (CHX) chase assay for NPC (Nup35, Nup54, Nup93a, and Nup160) and PNET (SUN1 and PNET2_A) proteins. Seedlings were treated with CHX for indicated time before protein was extracted and subject to immunoblot using anti-GFP and anti-actin antibodies. Protein quantification was normalized to time 0 in each sample and shown under immunoblots. Similar results were obtained three times.

Article Snippet: Protein extracts were separated by SDS–polyacrylamide gel electrophoresis, followed by immunoblot analysis using an anti-GFP antibody (1:5000 dilution Clontech, catalog no. 632381), an anti-hemagglutinin antibody (1:5000 dilution; Roche, catalog no. 11867431001), or streptavidin–horseradish peroxidase (1:10,000 dilution; Abcam, catalog no. 7403).

Techniques: Derivative Assay, Expressing, Functional Assay, Activity Assay, Transgenic Assay, Western Blot

Journal: Nature Communications

Article Title: Correction of the molecular phenotype of X-linked Dystonia-Parkinsonism reveals a non-canonical function of BRD4

doi: 10.1038/s41467-026-72319-6

Figure Lengend Snippet: a Schematic representation of the MG and MG-SVA minigene reporters. Exons are shown as boxes and numbered; the XDP-SVA insertion is indicated. The epitope recognized by the TAF1 antibody is indicated in pink; the GFP antibody is shown for reference in blue. Relative abundance of splicing junctions for canonical exons ( b ) and i32 ( c ) quantified by RT-qPCR for MG and MG-SVA. d Ratio of exon 35-mKATE2 to linker-exon 31 RT-qPCR products ( p = 0.0046). e Sashimi plot depicting splicing acceptors usage from exon 32 splicing donor site. Read count is depicted as read counts/1000. f Dot plot indicating the percentage of incorporation of the different minigene exons for the identified transcript isoforms. Error bars indicate Wilson 95% confidence intervals. g Immunoblot of TAF1 minigene-derived protein products using GFP and TAF1-specific antibodies. Truncated products in XDP-SVA-expressing cells are indicated with asterisks (*). Molecular weight is expressed in kDa. h Representative fluorescence microscopy images showing GFP and mKATE2 expression in MG- or MG-SVA-containing cells. Scale bars, 20 μm. Box plots ( b – d ) are generated with n = 3 biological replicates. Dot plot ( f ) is generated with n = 1. Source data are provided as a Source Data file.

Article Snippet: The antibody used included: GFP (Takara, cat. no. 632381, clone ID JL8, lot A8034133, 1:2000), TAF1 (from ref. , 1:1000), GAPDH (Millipore, cat. no. MAB374, clone ID 6C5, lot 4047566, 1:1500), BRD4 (Cell Signaling, cat. no. 13440, clone ID E2A7X, lot 10, 1:1000), Vinculin (Santa Cruz, cat. no. sc-73614, clone ID 7F9, lot H1721, 1:1000), CDK9 (Cell Signaling, cat. no. 2316, clone ID C12F7, lot 10, 1:1000), pol II Ser2-P (Cell Signaling, cat. no. 31262, clone ID E1Z3G, lot 1, 1:1000), cleaved PARP (Cell Signaling, cat. no. 9541, lot 22, 1:1000) and Caspase 3 (Cell Signaling, cat. no. 9662, lot 19, 1:500).

Techniques: Quantitative RT-PCR, Western Blot, Derivative Assay, Expressing, Molecular Weight, Fluorescence, Microscopy, Generated